Determining the dwell volume of an LC system is a useful activity. This technical note describes the theory behind dwell volume and how to measure it accurately for use in gradient method transfers between different instruments to maintain peak retention, selectivity and resolution.
The dwell volume or gradient delay volume is described as the system volume between the point at which eluents are combined in the pump mixer and the head of the column.
Dwell volumes differ considerably depending on the configuration of the instrument, the vendor, the volume of tubing, between binary or quaternary pumps and if any mixers are installed. Typical instrument dwell values can be seen in Table 1.
Table 1: Typical dwell volume values for different instrument manufacturers.>> Download the full Application Note as PDF
Newsletters
Receive the latest analytical scientist news, personalities, education, and career development – weekly to your inbox.
