LC analyses of sphingophospholipids often show peak tailing and loss of sensitivity and reproducibility. An improved method using YMC-Triart metal-free columns is presented in this app note.
Sphingolipids are major components forming biological membranes, and they serve as intracellular signaling molecules. It is important to measure the amount of these molecules in biological samples because they have massive influence on various metabolic diseases such as obesity, diabetes, and Alzheimer’s disease. However, in LC analysis of sphingophospholipids, such as sphingosine-1-phosphate (S1P) and ceramide-1-phosphate (C1P), a phosphate group in those molecules causes significant peak tailing, and thus loss of sensitivity and reproducibility. Recently, an improved method using a YMC-Triart C18 metal-free column was reported by Dr. Gowda et al. 1)
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