High-resolution, accurate-mass (HRAM) mass spectrometers (MS) are a class of MS instrumentation with capability to resolve complex sample matrix and to allow identification of compounds by measuring their accurate masses. HRAM have been used extensively for structural elucidation of unknown compounds primarily in chemical industry (impurity analysis) and biological researches (proteomics, metabolomics, lipidomics), but also increasingly in forensic science, food safety and environmental testing.
The key performance attribute that governs the ease and confidence of all HRAM applications is mass measurement accuracy (MMA) as it affords molecular specificity and reduction of false positive results. Understanding various factors affecting the MMA will help scientists conduct better analysis by taking effective measures to sustain stable MMA. The present article summarizes the headlines of the “mass accuracy white paper” that fully explains the fundamentals and principles of MMA. Topics include the fundamentals of ion statistics, tips on mass calibration, factors affecting mass calibration, and lock-mass correction.
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