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The Analytical Scientist / App Notes / 2019 / The Purification of Cannabidiol Using CPC

The Purification of Cannabidiol Using CPC

04/23/2019

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Recent changes in the legal status of Cannabis compounds for medicinal use, along with the decriminalization of marijuana in some locations, has led to increased interest in purification, formulation, and detection of CBD. Gilson has developed a rapid and reproducible method for purification using centrifugal partition chromatography (CPC).

The Cannabis sativa plant is rapidly gaining in popularity because of the medicinal application of the non-psychotropic component, cannabidiol (CBD), which can aid in treatment of conditions including: pain, inflammation, epilepsy, and cancer. Interest in the purification, formulation, and detection of CBD has increased rapidly due to the recent changes in the legal status of cannabis compounds for medicinal use. As a result, Gilson has developed a rapid and reproducible method for large-scale purification of CBD using the liquid-liquid chromatography technique, centrifugal partition chromatography (CPC). A liquid stationary phase is retained inside the CPC column by a centrifugal field while a non-miscible liquid is pumped through as the eluent. The separation of components in the sample is dependent on the phase volume ratio inside the column and the partition coefficient of the solutes in both phases.

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