Mycophenolic acid (Figure 1) is an active metabolite produced by the hydrolysis of the immunosuppressive agent Mycophenolate Mofetil in the body. It is considered important to control pharmacokinetic parameters such as AUC (area under the blood concentration-time curve) and trough concentration in order to prevent acute rejection after organ transplantation.
In this application note, ISOLUTE® SLE+ was used as a sample pretreatment column for mycophenolic acid determination in plasma using the principles of supported liquid extraction (SLE). UV based methods are commonly used to measure mycophenolic acid. In this application note, we have developed a method that can be used for both UV measurement and LC/ MS/MS measurement. LC/MS/MS assays are highly selective and less susceptible to the effects of other drugs used in combination during treatment.
ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquidliquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.
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