Raman spectroscopy combines high specificity with tremendous ease of use to identify unknown compounds. The principle of operation is simple: point and shoot a laser at your sample, collect the light scattered back with a spectrometer, and you get a Raman spectrum which is as detailed and unique to the sample as a fingerprint – perfect for identification. And since no sample preparation is required, it is no wonder that Raman spectroscopy is becoming the goto tool for instantaneous identification of unknowns. But how does that ‘identification’ actually occur? In this tech note, we’ll introduce both the theory and best practices behind the most common method, ‘library matching’, and explain how a basic match can be performed easily using any of our Raman spectrometers in our ENLIGHTEN™ spectroscopy software. If you want a simple way to get started with Raman library matching and build a custom library perfectly tailored to your application, this is the resource for you!
To perform accurate Raman library matching, you must first start with a good-quality Raman spectrum. This calls for a high sensitivity spectrometer with good signal-to-noise ratio to reduce errors in matching. Adequate spectral resolution is also important, as it affects peak clarity and match accuracy. A spectrometer resolution of 8-15 cm-1 is sufficient for many applications, depending on the complexity of the sample and the quantity and similarity of the compounds to be differentiated. Choosing the appropriate excitation laser wavelength will ensure minimal fluorescence interference, and optimizing the laser interface with the sample will maximize signal. Learn more about choosing a portable Raman spectrometer and laser excitation wavelength.
>> Download the Application Note as a PDF
Newsletters
Receive the latest pathologist news, personalities, education, and career development – weekly to your inbox.
