Accelerate Method Development using Fast Screening

Introduction

It is now well-known that mobile phase components (i.e. additives and solvents) play a major role in ionization efficiency. Laboratory facing challenges in fast method development and high sensitivity are often condemned to use generic mobile phases and to invest in expensive high-end mass spectrometers. Furthermore, recent developments in HPLC columns allow more flexibility in the use of acidic or basic additives as well as viscous solvents. In this study, we propose a rapid and systematic methodology to quickly optimize HPLC mobile phase recipe from a MS sensitivity point of view.