Accelerating UHPLC/HPLC method development and maximising chromatographic selectivity with novel stationary phase chemistries
In all modern analytical laboratories it is increasingly important to be able to carry out accurate and reproducible HPLC analyses, with excellent turnaround time and throughput. For those laboratories that must develop, validate and use methods over a significant period of time, or those that must transfer methods to other laboratories around the world, it is a practical business advantage to be able to develop robust, rugged methods more quickly. For both situations, it can be beneficial to have a diverse group of column chemistries, from which you can choose and use to explore chromatographic selectivity.
contributed by VWR |
Two fundamental requirements for obtaining accurate, and reproducible results in HPLC and UHPLC, are excellent peak shape and adequate resolution between the analytes. Good symmetrical peak shape in Reversed-Phase Liquid Chromatography (RPLC) comes from an appropriate choice of column, mobile phase, and a variety of other parameters including sample solvent, injection volume, buffer type and strength. A high quality column made from ultra-pure, low-metal-content silica, can play a key role in minimising secondary interactions of acidic and basic analytes with the stationary phase, which can lead to tailing and fronting peaks. Combining a series of stable, unique bonding chemistries with ultra-pure, silica particles, can make the method development process faster, more comprehensive and more effective. Moreover, by choosing shorter column geometries with smaller particle sizes, such as 1,7; 2 and 3 μm particles, comprehensive method development and evaluation of the ‘chromatographic selectivity space’ for analytes can be accomplished faster.
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