Fields & Applications Proteomics, Mass Spectrometry

Achieving robust, accurate TMT quantitation efficiency with Tribrid technology

contributed by Thermo Fisher Scientific | 11/28/2017

To provide conclusive arguments to adopt the revolutionary Tribrid technology for a high throughput quantitative proteomics workflow.

Multichannel, isobaric tagging offers confident and reliable quantitation information for biological experiments. The multiplexing potential of the Thermo Scientific™ TMT10plex™ Isobaric Mass Tag Labelling Kit (Proteome Sciences,^® commercially available from Thermo Fisher Scientific) is fully maximized when used with a SPS MS³-based method exclusive to Thermo Scientific™ Tribrid™ mass spectrometers. Uniquely designed Tribrid MS systems are capable of performing synchronous precursor selection (SPS) multi-notch MS³ quantitative analyses to improve experimental throughput and yet still providing the depth and coverage, eliminating issues encountered with TMT ratio distortions caused by interfering ions, without neglecting sensitivity that is often observed. Other commercially available mass analyzers have limitations to handle highly multiplexed isobaric tags as they lack the analytical performance features, i.e. high resolution and multiple analyzers for concurrent isolation and detection of ions, to accomplish a SPS MS³-type technique for quantitation.

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