A highlight of the capabilities of the Thermo Scientific™ Chromeleon™ software in performing and simplifying complex calculation using advanced quantitation tools. These tools are extremely beneficial for all those applications that require the use of multiple ISTDs. Dioxin analysis is used here as an example to highlight calculation features such as multiple ISTDs with variable amounts and custom variables setting.
Introduction
Most chromatographic assays are straightforward when it comes to the calibration of standard solutions and subsequent calculation of sample results. The most commonly used techniques include single or multipoint calibrations from external or internal standards. These can be easily set up by most chromatographers in Thermo Scientific™ Chromeleon™ 7 Chromatography Data System (CDS) software.
For more complex calibration methods, such as those used in the analysis of dioxins, polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), polybrominated biphenyls (PBBs), polyfluorinated compounds (PFCs), and other halogenated flame retardents, Chromeleon CDS supports multiple internal standard (ISTD) components with variable amount values, eliminating the need to perform the calculations outside the software.
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