Fields & Applications Forensics, Sample Preparation

An In-Well Hydrolysis Solid Phase Extraction Method Using EVOLUTE® HYDRO CX for 96 Licit and Illicit Drugs of Abuse from Urine Prior to UPLC-MS/MS

contributed by Biotage | 10/10/2017

This application note describes the extraction of 96 licit and illicit drugs of abuse from urine prior to UPLC-MS/MS analysis using EVOLUTE^® HYDRO CX 96-well plates.

EVOLUTE^® HYDRO CX plates offer an efficient way to perform hydrolysis in the well of the extraction plate. This method provides high analyte recovery, reduced extraction time due to the elimination of a sample transfer step as well as the elimination of the column conditioning and equilibration steps, and a reduced risk for sample carryover or cross-contamination due to the elimination of the sample transfer step.

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