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Analysis of benzodiazepines and their metabolites by UHPLC-MS/MS

Overview

The analysis of benzodiazepines in biological fluids is important to clinicians and forensic toxicologists because of their sedative, hypnotic, antianxiety, antiepileptic and muscle relaxant properties. It is challenging to analyse a mixture of etizolam, triazolam, and their metabolites because their chemical structures, molecular weights and fragmentation patterns during mass spectrometry are very similar.

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