Targeted metabolomics methods provide a sensitive and precise measurement of metabolites with a large dynamic range. Previously described is a HILIC polar metabolite workflow using Bravo Sample Prep Platform with cells or plasma, a 1290 Infinity II Bio LC for improved performance of metal sensitive analytes, and a 6495 LC/TQ mass spectrometer with a database of ~500 polar metabolites and retention times (Figure 1).1 The speed of the 6495 LC/TQ allows for hundreds of analytes in both positive and negative ion mode to be analyzed with precision in the same injection. This workflow and database can be deployed in several ways, from metabolite pathway discovery (profiling), to semi quantitative analysis of hundreds of analytes in a sample, or for absolute quantitation using isotopically labeled internal standards.
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