Application of Differential Ion Mobility Mass Spectrometry to Peptide Quantitation

contributed by SCIEX | 07/11/2014

Using SelexION™ Differential Mobility Separation Technology for better selectivity for peptides in complex mixtures on the AB SCIEX Triple Quad™ 5500 LC/MS/MS System

Reduced recovery, low sensitivity – The adsorptive properties and/or polarity of peptides can compromise recovery, and interferences from biological matrices can negatively impact sensitivity and selectivity.
Limited quantitation range – Poor MS/MS sensitivity combined with often poor selectivity can compromise the desired lower limits of quantitation (LLOQ).
Limited MRM selectivity – MRM approaches and efficient UHPLC separations may not provide adequate signal-to-noise ratios at LLOQ due to isobaric interferences or high baseline noise.

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