Automated extraction of carboxy-THC from urine prior CG/MS analysis

Automated Extraction of 11-nor-9-Carboxy-Δ⁹- THC from Hydrolyzed Urine Using EVOLUTE^® EXPRESS ABN Prior to GC/MS Analysis

Introduction

This application note describes the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. The method was automated using Biotage^® ExtraheraTM, configured for use with EVOLUTE EXPRESS ABN columns.

The method delivers clean extracts and analyte recovery of 90% with RSD lower than 10% for carboxy-THC and its deuterated internal standard. Using the Biotage^® Extrahera^TM, 24 samples were extracted in approximately 38 minutes. The limit of quantitation of 2 ng/mL is below the SAMHSA/ EWDTS confirmation cut off of 15 ng/mL for workplace testing applications.

The EVOLUTE^® EXPRESS range of SPE columns and 96-well plates combine powerful EVOLUTE sorbent chemistry with innovative features that provide robust, reliable SPE, giving ultra-pure extracts and high analyte recoveries. With improved flow-through characteristics and by eliminating the need for conditioning and equilibration, samples can be prepared much faster using the simplified Load-Wash-Elute procedure.

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