Introduction
This application note describes the fully-automated extraction of opiates from acid-hydrolyzed urine and non-hydrolyzed urine prior to GC/MS analysis. The methods were automated using Biotage® Extrahera™ configured for use with ISOLUTE® SLE+ supported liquid extraction columns.
The simple sample preparation procedure delivers clean extracts and analyte recoveries of greater than 74% with RSDs lower than 10%. Using the Biotage® Extrahera™, 24 hydrolyzed samples were extracted in under 31 minutes and 24 non-hydrolyzed samples were extracted in under 36 minutes. The limits of quantitation, described in Table 2, all meet or exceed the sensitivity requirements set by SAMHSA and EWDTS for workplace testing applications.
ISOLUTE® SLE supported liquid extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.
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