Cookies

Like most websites The Analytical Scientist uses cookies. In order to deliver a personalized, responsive service and to improve the site, we remember and store information about how you use it. Learn more.

Automated Hydrolysis, Extraction and Determination of Opioids in Urine using a Novel Robotic Autosampler and LC-MS/MS Platform

Automated Hydrolysis, Extraction and Determination of Opioids in Urine using a Novel Robotic Autosampler and LC-MS/MS Platform

Abstract

The Opioid Epidemic continues to increase throughout the United States. According to the CDC, 66 % of all drug overdose deaths in 2016 involved an opioid [1]. This calculates to roughly 116 deaths every day from opioid related overdoses. After becoming addicted to prescription opioids, users may unfortunately turn to illicit alternatives such as heroin. To compound the issue, heroin has increasingly been found to be mixed with other synthetic opioids such as fentanyl, which is 100 times more potent than morphine. In order to respond effectively to this epidemic, forensic, health care, and law enforcement scientists need access to fast methods for assessing and monitoring which opioids are involved.

Automating the entire hydrolysis, extraction, and subsequent analysis by LC-MS/MS provides the critically needed high throughput analysis for opioids in urine. Using the novel GERSTEL MPS robotic autosampler, syringe transfer of all liquids involved in the enzymatic hydrolysis procedure, controlled incubation of the samples for a defi ned period of time, as well as extraction of the subsequent hydrolyzed urine samples using dispersive solid phase extraction were performed. The resulting eluents from the automated extractions were then introduced into the new Agilent Ultivo LC-MS/MS instrument.

Read the full article now

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE and always will be!

Login

Or register now - it’s free and always will be!

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine
Register

Or Login via Social Media

By clicking on any of the above social media links, you are agreeing to our Privacy Notice.

Newsletter

Send me the latest from The Analytical Scientist.

Sign up now

Related Articles

Techniques & Tools Capillary Electrophoresis

Gurus of Capillary Electrophoresis

| Cari Sänger-van de Griend, Hermann Wätzig, Michael Breadmore

Fields & Applications Food, Beverage & Agriculture

Food for Thought

| Sponsored by GE Healthcare

Fields & Applications Technology

The Innovators 2018

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine

Register