Automated NGS Library Prep: Ligation Efficiency of NxSeq® Sample Preparation Using the Gilson PIPETMAX® 268

Introduction

As the number of samples for sequencing increases, there is a need for library prep automation that matches the throughput of the next generation sequencing (NGS) instruments while not requiring an investment equal to the sequencer itself. By utilizing the Gilson PIPETMAX^® 268, a novel NGS sample prep chemistry was automated to reduce the time to achieve the final prepared library, increasing the efficiency of the method and consistency of the resulting DNA library to be sequenced.