Conexiant
Login
  • The Analytical Scientist
  • The Cannabis Scientist
  • The Medicine Maker
  • The Ophthalmologist
  • The Pathologist
  • The Traditional Scientist
The Analytical Scientist
  • Explore

    Explore

    • Latest
    • News & Research
    • Trends & Challenges
    • Keynote Interviews
    • Opinion & Personal Narratives
    • Product Profiles
    • App Notes

    Featured Topics

    • Mass Spectrometry
    • Chromatography
    • Spectroscopy

    Issues

    • Latest Issue
    • Archive
  • Topics

    Techniques & Tools

    • Mass Spectrometry
    • Chromatography
    • Spectroscopy
    • Microscopy
    • Sensors
    • Data and AI

    • View All Topics

    Applications & Fields

    • Clinical
    • Environmental
    • Food, Beverage & Agriculture
    • Pharma and Biopharma
    • Omics
    • Forensics
  • People & Profiles

    People & Profiles

    • Power List
    • Voices in the Community
    • Sitting Down With
    • Authors & Contributors
  • Business & Education

    Business & Education

    • Innovation
    • Business & Entrepreneurship
    • Career Pathways
  • Events
    • Live Events
    • Webinars
  • Multimedia
    • Video
Subscribe
Subscribe

False

The Analytical Scientist / App Notes / 2014 / Biomonitoring of Phthalate Metabolites in Human Urine using ISOLUTE® ENV+ Columns Prior to LC-MS/MS

Biomonitoring of Phthalate Metabolites in Human Urine using ISOLUTE® ENV+ Columns Prior to LC-MS/MS

09/24/2014

Share

Featured Image

This application note describes the extraction of nine phthalate metabolites from human urine using ISOLUTE® ENV+ solid phase extraction columns.

Introduction

Phthalates are plasticizers used in industry to adjust the mechanical (and sometimes barrier) properties of plastics in consumer products and packaging. Their ubiquitous presence in our everyday lives constantly presents threats of low level exposure through inhalation or ingestion. Thus, large biomonitoring studies including the US National Health and Nutrition Examination Survey (NHANES) have screened for phthalates since 1999. Because phthalates themselves are difficult to eliminate from sampling and processing materials, including laboratory ware and instruments, analysis of phthalates in human samples have focused on their monoester metabolites. Monoethyl phthalate (MEP), monobutyl phthalate (MBP), monobenzyl phthalate (MBzP) and mono (2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) have been constantly detected in human urine since the first NHANES survey of phthalates in 1999. Phthalates exposure has been associated with decreased anogenital distance, lower sperm count, cryptorchidism and hypospadias among other clinical endpoints in humans.

10414-app-note-biotage-logo

To facilitate the high throughput population screening of phthalate metabolites, sample preparation methods need to be simple, sensitive and robust to mitigate matrix suppression and instrument down time common to many dilute and shoot approaches to mass spectrometry. Thus, a solid phase extraction procedure was developed for these analytes. This application note details the optimization strategy for nine phthalate metabolites. Proof-of-concept for this sample preparation method was determined on a set of real patient samples (n=5). The results were in general agreement with previously reported concentration ranges for these compounds. It is anticipated that this method will have significant impact in environmental biomonitoring strategies for these analytes.

10414-app-note-biotage-mainFigure 1. Structures of the target analytes in the phthalate metabolites panel.
Analytes

Monomethyl phthalate (MMP); Monoethyl phthalate (MEP); Monobutyl phthalate (MBP); Monobenzyl phthalate (MBzP); Monohexyl phthalate (MHxP); Mono (2-ethylhexyl) phthalate (MEHP); Mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP); Mono (2-ethyl-5-carboxypentyl) phthalate (MECPP); Monoisononyl phthalate (MiNP).

>> Download the full Application Note as PDF

Newsletters

Receive the latest pathologist news, personalities, education, and career development – weekly to your inbox.

Newsletter Signup Image

Explore More in Pathology

Dive deeper into the world of pathology. Explore the latest articles, case studies, expert insights, and groundbreaking research.

False

Advertisement

Recommended

False

Related Content

Comprehensive Analysis of Drug Residues from a Confiscated Pipe
Comprehensive Analysis of Drug Residues from a Confiscated Pipe

May 1, 2014

This application note shows the utility of high resolution mass spectrometry with soft ionization to facilitate identification of unknown compounds which were present in extracted residues from a confiscated pipe...

Extraction and Analysis of Neonicotinoid Pesticides from Flower Blossoms
Extraction and Analysis of Neonicotinoid Pesticides from Flower Blossoms

May 6, 2014

In this application, the Quick, Easy, Cheap, Effective, Rugged, Safe (QuEChERS) approach was used to develop an extraction and cleanup method for the analysis of seven neonicotinoid pesticides in flower blossoms...

Ultrapure water: LC-MS suitability tests
Ultrapure water: LC-MS suitability tests

May 7, 2014

Fresh ultrapure water used in all the experiments was produced from a Milli-Q® water purification system fed by an Elix system...

GC/MS analysis of a complex sample in DCM using hydrogen carrier gas
GC/MS analysis of a complex sample in DCM using hydrogen carrier gas

May 8, 2014

This application note aims to demonstrate the injection of a complex, 76-component sample diluted in Dichloromethane (DCM) using hydrogen carrier gas...

False

The Analytical Scientist
Subscribe

About

  • About Us
  • Work at Conexiant Europe
  • Terms and Conditions
  • Privacy Policy
  • Advertise With Us
  • Contact Us

Copyright © 2025 Texere Publishing Limited (trading as Conexiant), with registered number 08113419 whose registered office is at Booths No. 1, Booths Park, Chelford Road, Knutsford, England, WA16 8GS.