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A Simple Step-by-Step Protocol for HILIC Method Development

ABSTRACT

The ever increasing demands on chromatographers to rapidly produce fit for purpose separations requires more efficient and methodical working processes. Method development workflows are generally well established for reversed phase liquid chromatography. Hydrophillic Interaction Liquid Chromatography (HILIC) is less well understood practically and mechanistically, meaning that the method development process tends to be less systematic and well defined. This ACE Knowledge Note details a simple, rationally designed protocol for HILIC method development using the three ACE HILIC phases.

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