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Cannabinoid Potency Testing by HPLC according to DAB Monography for Cannabis Flower with use of Co-Injection function

Due to the use of different, strong organic solvents for standard dilution and extraction the analytical method for potency testing in cannabis flower according to DAB 2018 results in peak dispersion and different chromatographic retention for sample and standard solutions. Co-injection of water reduces these differences, and significantly improves peak shape and therefore resolution. The i-series Plus compact (U)HPLC system offers the automated co-injection function.

Introduction

In the German Pharmacopoeia 2018 (DAB 2018) the Federal Institute for Drugs and Medical Devices (BfArM) publishes a revised monograph for cannabis flower (Cannabisflos) [1]. Since there is no equivalent monograph in the European Pharmacopeia (EP), currently the method described in the DAB 2018 depicts the obligatory procedure for potency testing of cannabinoids in cannabis flower in the EU [2].

The monograph constitutes the official pharmaceutical regulation according to the law on drugs (AMG) and will be considered for decisions made by the responsible authority.

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