Characterisation Studies of PEGylated Lysozyme

contributed by Tosoh | 09/29/2014

PEGylation, the process by which polyethylene glycol (PEG) chains are attached to protein and peptide drugs is a common practice in the development of biopharmaceuticals to prolong serum half-life and improve pharmacokinetics of a drug. There is increasing demand for chromatographic methods to separate the modified isoforms from the native protein. This application note describes the use of size exclusion and ion exchange chromatography for the characterization of PEGylated lysozyme.

Chemical modification of therapeutic proteins in order to enhance their biological activity is of increasing interest. One of the most frequently used protein modification method is the covalent attachment of poly (ethylene glycol) which is called PEGylation. This polymeric modification changes the biochemical and physicochemical properties of the protein, which decreases the in vivo clearance rate and reduces toxicity and immunogenicity of therapeutic proteins.

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