Column Equilibration in HILIC Mode

contributed by Ace | 11/20/2019

ABSTRACT

In order to obtain stable and reproducible retention times, it is essential to fully equilibrate HILIC columns with mobile phase prior to analysis to ensure a stable adsorbed water layer exists at the stationary phase surface. Similarly, after performing a gradient run, the column requires re-equilibration to the gradient starting conditions and formation of a stable adsorbed water layer.

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