Conexiant
Login
  • The Analytical Scientist
  • The Cannabis Scientist
  • The Medicine Maker
  • The Ophthalmologist
  • The Pathologist
  • The Traditional Scientist
The Analytical Scientist
  • Explore

    Explore

    • Latest
    • News & Research
    • Trends & Challenges
    • Keynote Interviews
    • Opinion & Personal Narratives
    • Product Profiles
    • App Notes

    Featured Topics

    • Mass Spectrometry
    • Chromatography
    • Spectroscopy

    Issues

    • Latest Issue
    • Archive
  • Topics

    Techniques & Tools

    • Mass Spectrometry
    • Chromatography
    • Spectroscopy
    • Microscopy
    • Sensors
    • Data and AI

    • View All Topics

    Applications & Fields

    • Clinical
    • Environmental
    • Food, Beverage & Agriculture
    • Pharma and Biopharma
    • Omics
    • Forensics
  • People & Profiles

    People & Profiles

    • Power List
    • Voices in the Community
    • Sitting Down With
    • Authors & Contributors
  • Business & Education

    Business & Education

    • Innovation
    • Business & Entrepreneurship
    • Career Pathways
  • Events
    • Live Events
    • Webinars
  • Multimedia
    • Video
Subscribe
Subscribe

False

The Analytical Scientist / App Notes / 2013 / Comparison of Two Beer Samples by GCxGC-TOFMS

Comparison of Two Beer Samples by GCxGC-TOFMS

06/17/2013

Share

Featured Image
Introduction

The "COMPARE" feature, available in the Data Processing Method of ChromaTOF , is a single point calibration curve generated from a "Reference" sample, used to compare the Reference sample against a target sample. In this application note, the COMPARE feature will be used to compare two different samples of beer analyzed by SBSEGCxGC- TOFMS. The objective is to identify variations in twenty targeted compounds between the two samples. The Reference sample is of beer stored cold. The other sample is of the same beer that had been stored at an elevated temperature. For the remainder of this work, the sample from which the reference standard is made will be referred to as the "Reference" and the sample being compared to the reference standard will be called the "Sample". 

Logo of LECO
Instruments and Methods

In this study, measurements were made with a LECO Pegasus 4D GCxGC-TOFMS system. This system consists of an Agilent 6890 gas chromatograph equipped with a LECO dual-jet thermal modulator between the primary and secondary columns and a LECO Pegasus IV Time-of- Flight Mass Spectrometer (TOFMS) as a detector. For this study, the primary analytical column was a GERSTELMACH LTM 10.0 m x 0.18 mm ID x 0.20 μm df Rtx-5. The secondary column was a 1.00 m x 0.10 mm ID x 0.10 μm df DB-17ms and was housed in the GC oven. The temperature program for the LTM started at 30 C held for 2 minutes then ramped at 4 C/minute to 230 C and then held for 10 minutes. The column temperature offset for this study was +20 C. The modulator temperature offset for this study was +25 C. Helium was used as the carrier gas at a corrected constant flow of 1.5 mL/minute. The transfer line to the TOFMS consisted of the last 20 cm of the analytical column and was kept at 280 C. Aliquots of 10 mL were prepared from each sample. The aliquots were placed in 10 mL headspace vials, each containing a 10 mm x 0.5 mm GERSTEL PDMS stir bar and extracted on a stir plate at 900 rpm and 25°C for 120 minutes. Upon completion of the extraction, the stir bar was removed, rinsed with DI water and placed in a GERSTEL Twister Desorption Unit (TDU) tube for analysis. The tube containing the stir bar is loaded into the TDU and the GERSTEL Cooled Inlet System (CIS 4) is cooled. The TDU is then heated, and the analytes are desorbed from the stir bar. The analytes are then trapped in the cooled CIS 4. The CIS 4 is then heated to desorb the analytes onto theGC column. The TDU was operated in splitless mode. Its initial temperature was 20 C and was held at this temperature for an equilibration time of 30 seconds. It was then heated to 250 C at a rate of 700 C/minute and held at this temperature for 120 seconds. The CIS 4 was cooled to a temperature of -130 C. After a 6 second delay, it was heated to 260 C at a rate of 10 C/second and held for 120 seconds. 

Results

The cold beer sample was data processed with a Signalto- Noise ratio (S/N) cut-off of 0 and a required spectral similarity against the NIST 05 library of 700. A "Reference" was then created and the sample was added as the standard for the Reference. The list of compounds in the Peak Table was reduced to include only the twenty targeted components.

  1. N-Nitrosodimethylamine
  2. 2-Hexanone
  3. Methane sulfonic anhydride
  4. Dimethyl trisulfide
  5. 1-Octanol
  6. Decanal
  7. Azulene
  8. 4-Benzyloxybenzonitrile
  9. Octanoic acid, ethyl ester
  10. 1,2,3,4-tetrahydro-1,1,6-trimethyl-naphthalene
  11. Isopentyl hexanoate
  12. Acetic acid, 2-phenylethyl ester
  13. Benzenecarboxylic acid
  14. Benzenepropanoic acid, ethyl ester
  15. Citronellyl acetate
  16. 3-methyl tridecane
  17. 3-phenyl-2-propenoic acid, ethyl ester
  18. 5-butyldihydro-2(3H)-furanone
  19. d-Cadinene
  20. Hexadecane

The user-specified criteria that must be set in the Reference are as follows. "R.T. Deviation(s)"(±)
the allowable retention time deviation in the 2nd dimension for a Match  "1st Dimension Retention Deviation"(±)
the allowable retention time deviation in the 1st dimension for a Match (use multiples of the Modulation Period)  "Match Threshold"
the minimum spectral similarity for a Match "S/N Threshold"
the minimum S/N for a Match (NOTE: This S/N uses the Quant S/N obtained from the integrated peak, which is different than the Qualitative S/N used by Peak Find in the Data Processing Method) and "Tolerance", which is the allowable variation in Concentration (area in this case, the units are user defined) to be considered a Match. 

>> Download the full Application Note as PDF

Newsletters

Receive the latest analytical scientist news, personalities, education, and career development – weekly to your inbox.

Newsletter Signup Image

Explore More in Analytical Science

Dive deeper into the analytical science. Explore the latest articles, case studies, expert insights, and groundbreaking research.

False

Advertisement

Recommended

False

Related Content

Extraction of Multiple Mycotoxins From Grain Using ISOLUTE<sup>®</sup> Myco prior to LC-MS/MS Analysis
Extraction of Multiple Mycotoxins From Grain Using ISOLUTE<sup>®</sup> Myco prior to LC-MS/MS Analysis

April 25, 2013

This application note describes a Solid Phase Extraction (SPE) protocol for the extraction of a range of mycotoxins from wheat flour, wheat, maize and barley using ISOLUTE® Myco with LC-MS/MS...

Analysis of 18 Polycyclic Aromatic Hydrocarbons in Soil Using the QuEChERS Method
Analysis of 18 Polycyclic Aromatic Hydrocarbons in Soil Using the QuEChERS Method

April 29, 2013

The use of QuEChERS dispersive SPE as a simple, fast, and quantitative sample preparation method is demonstrated for the GC-MS analysis of 18 polycyclic aromatic hydrocarbons (PAHs) in soil...

Analysis of Low Level Pyrethroid Pesticides in Water
Analysis of Low Level Pyrethroid Pesticides in Water

April 29, 2013

A method for the determination of pyrethroids in water at ultra-low-level concentrations of 0...

Determination of Phthalates in Drinking Water by UHPLC with UV Detection
Determination of Phthalates in Drinking Water by UHPLC with UV Detection

April 29, 2013

To develop an efficient high-performance liquid chromatography (HPLC) method for the simultaneous determination of 19 phthalate compounds in drinking water...

False

The Analytical Scientist
Subscribe

About

  • About Us
  • Work at Conexiant Europe
  • Terms and Conditions
  • Privacy Policy
  • Advertise With Us
  • Contact Us

Copyright © 2025 Texere Publishing Limited (trading as Conexiant), with registered number 08113419 whose registered office is at Booths No. 1, Booths Park, Chelford Road, Knutsford, England, WA16 8GS.