Comprehensive 2D-LC Analysis of Chinese Herbal Medicine

contributed by Agilent Technologies | 12/02/2014

Achieving the highest chromatographic resolution using the Agilent 1290 Infinity 2D-LC solution.

Chinese herbal medicine (CHM) is one aspect of traditional Chinese medicine (TCM) and uses single plants or preparations of several plants. The effectiveness of CHM depends on the synergistic effects of multiple components in the plants. The plants used in CHM present extremely complex samples. Therefore, comprehensive twodimensional liquid chromatography (comprehensive 2D-LC) is the method of choice for their analysis.

For comprehensive 2D-LC analysis, samples of the plants were prepared as decoctions. It can be expected that decoctions of plants used in CHM contain a range of very polar compounds besides many less polar compounds. For this reason, an Agilent ZORBAX SB-Aq column was chosen for the first-dimension separation because it retains hydrophilic compounds and can be run under 100% aqueous conditions. Further, methanol showed better separation for the ingredients of mori ramulus, as well as adding 0.1% formic acid to both eluents. The gradient was run from 0 to 95% methanol over a duration of 80 minutes.

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