Confident O-glycosylation Site Identification for ENBREL (etanercept) Using the ECD Functionality of SELECT SERIES Cyclic IMS System

contributed by Waters | 01/29/2024

Characterization of glycosylation on the glycopeptide level can be challenging with traditional peptide mapping methods that employ reversed-phase liquid chromatography coupled with tandem mass spectrometry (RPLC- MS/MS or MS^E). These methods typically employ collision induced dissociation (CID) as the fragmentation technique, which has limitations for site specific analysis of labile modifications. The use of Electron Capture Dissociation (ECD) enables glycan moieties to be retained on the peptide backbone, and allow the site to be confidently assigned using the resulting fragment ions. In this study, we demonstrate the utility of ECD on the SELECT SERIES Cyclic IMS instrument for unambiguous assignment of glycosylation site locations for 11 O- glycopeptide species of ENBREL (etanercept).

ECD fragmentation generates critical site-specific O-glycosylation information Improved sensitivity, resolution, and IMS capabilities of the SELECT SERIES Cyclic IMS instrument provide comprehensive analytical tools to unravel this complex set of biotherapeutic attributes.

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE!

Email*

Choose a password*

I have read and understand the Privacy Notice *

Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

Receive communication from The Cannabis Scientist I want to stay up to date with the Mass Spectrometry field I want to stay up to date with the Spectroscopy field

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.