Creating the Correct Environment for Analysing Reducing Sugars with the ACE NH2

Abstract

Sugars are carbohydrates composed of carbon, hydrogen and oxygen. In cyclic form, they are capable of forming isomers known as anomers at the acetal carbon (known as the anomeric carbon). Anomerization is the conversion of one anomer to the other and readily occurs whilst in solution. Under non-ideal chromatographic conditions, the anomeric forms can be observed as two peaks. This ACE Knowledge Note explains the anomeric process and how to simply create the correct environment for analysing reducing sugars using ACE Excel NH₂.

Introduction

In sugar chemistry, all monosaccharides and some disaccharides are reducing sugars.

Reducing sugars contain an anomic carbon that is able to convert between the α- and β-anomeric forms. A non-reducing sugar is one which has used the anomeric carbons to form the glycosidic bond and therefore cannot interconvert.

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