Conexiant
Login
  • The Analytical Scientist
  • The Cannabis Scientist
  • The Medicine Maker
  • The Ophthalmologist
  • The Pathologist
  • The Traditional Scientist
The Analytical Scientist
  • Explore

    Explore

    • Latest
    • News & Research
    • Trends & Challenges
    • Keynote Interviews
    • Opinion & Personal Narratives
    • Product Profiles
    • App Notes

    Featured Topics

    • Mass Spectrometry
    • Chromatography
    • Spectroscopy

    Issues

    • Latest Issue
    • Archive
  • Topics

    Techniques & Tools

    • Mass Spectrometry
    • Chromatography
    • Spectroscopy
    • Microscopy
    • Sensors
    • Data and AI

    • View All Topics

    Applications & Fields

    • Clinical
    • Environmental
    • Food, Beverage & Agriculture
    • Pharma and Biopharma
    • Omics
    • Forensics
  • People & Profiles

    People & Profiles

    • Power List
    • Voices in the Community
    • Sitting Down With
    • Authors & Contributors
  • Business & Education

    Business & Education

    • Innovation
    • Business & Entrepreneurship
    • Career Pathways
  • Events
    • Live Events
    • Webinars
  • Multimedia
    • Video
Subscribe
Subscribe

False

The Analytical Scientist / App Notes / 2019 / Determination of Organic Compounds in Drinking Water by Empore™ C18 SPE Disks and GC-MS with EPA Method 525.2

Determination of Organic Compounds in Drinking Water by Empore™ C18 SPE Disks and GC-MS with EPA Method 525.2

10/10/2019

Share

Featured Image

Introduction

The target analyte list for EPA Method 525.2 is comprised of 110 compounds that are representative of four organic compound classes as pesticides, polynuclear aromatic hydrocarbons, PCBs, phthalates and adipates. Method detection limits (MDLs), as published in the method, ranges from 0.03-2.4μg/L and the recovery rate varies from 20 – 180% for each individual compound. Furthermore, after averaging each compound within the four compound classes, the averaged recovery rate for each class is:

  • Pesticides                          108%
  • PCBs                                  108%
  • Phthalates & Adipates     116%
  • PAHs                                  112%

EPA Method 525.2 specified SPE disks as the sample preparation tool for the cleanup and concentration of organic contaminants from drinking water samples1,2. There are two challenges in the methods in the sample preparation as (1) large sampled volume at 1 liter, and (2) low pH at 2. EmporeTM C18 disks can consistently tackle with these challenges without loss of C18 phase from the silica support in the disks. EPA Method 525.2 specially warned that stripping C18 phase in the extraction disk packing will complicate the chromatographic analysis with high background, which could obscure the testing results on compounds of interests.

In this application note, a one-liter water sample was passed through a 47mm C18 Empore™ disk and eluted with ethyl acetate and methylene chloride under negative pressure. Then the extract was dried and reduced in volume down to 1.0 mL and future analyzed by GC/MS.

The validation data presented herein was determined on three separate lots of C18 disks. MDLs were not discussed as it relies on the GC/MS instrument setup. The recovery rate and RSDs were the key focus. In addition to the analytes listed in EPA Method 525.2, recovery data for an extended list of analytes is also studied.

>> Download the full Application Note as PDF

Newsletters

Receive the latest analytical scientist news, personalities, education, and career development – weekly to your inbox.

Newsletter Signup Image

Explore More in Analytical Science

Dive deeper into the analytical science. Explore the latest articles, case studies, expert insights, and groundbreaking research.

False

Advertisement

Recommended

False

Related Content

Ionic impurities in drug products – USP proposes new ion chromatography method
Ionic impurities in drug products – USP proposes new ion chromatography method

January 21, 2019

Chloride and sulfate are common impurities present in drug substances and drug products...

Decoding Dangerous Drinks with a Spectral Sensor
Decoding Dangerous Drinks with a Spectral Sensor

January 24, 2019

Have you ever heard that moonshine will make you go blind? Today, even your favorite, top-shelf liquor may be just as much of a risk...

Volume Fraction Determination of Ethanol in Splash-Blended Fuel Mixture
Volume Fraction Determination of Ethanol in Splash-Blended Fuel Mixture

January 24, 2019

While electric vehicles are becoming more mainstream the use of traditional gasoline engines will have a place in society for decades to come...

Cleaning Up IPA Production with Stage-by-Stage MIR Analysis
Cleaning Up IPA Production with Stage-by-Stage MIR Analysis

January 24, 2019

2-Propanol is one of the most common solvents in the world, with over 2 million tons produced in 2003 (Science)...

False

The Analytical Scientist
Subscribe

About

  • About Us
  • Work at Conexiant Europe
  • Terms and Conditions
  • Privacy Policy
  • Advertise With Us
  • Contact Us

Copyright © 2025 Texere Publishing Limited (trading as Conexiant), with registered number 08113419 whose registered office is at Booths No. 1, Booths Park, Chelford Road, Knutsford, England, WA16 8GS.