Determination of Taxanes in Taxus sp. with the Agilent 1290 Infinity 2D-LC Solution

contributed by Agilent Technologies | 02/18/2014

Taxane standard solutions and Taxus sp. extracts were analyzed with comprehensive two-dimensional liquid chromatography (LC×LC) using the Agilent 1290 Infinity 2D-LC solution with DAD and MS detection. Reversed-phase liquid chromatography was applied in both the first and second dimension. Orthogonality was achieved by using different column chemistries and mobile phases. Qualitative and quantitative aspects of the method were investigated and tested with real samples.

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