Direct Analysis of Intact Proteins Using the Advion Touch ExpressTM

Open Port Sampling Interface (OPSI) with the expression Compact Mass Spectrometer (CMS)

Touch Express OPSI coupled to the expression CMS provides fast benchtop analysis of intact proteins. Here, 2 μL of myoglobin (horse) at a concentration of 1 mg/mL in 10 mM ammonium acetate was used and deposited directly at the open port by pipette tip.

Following sample analysis, a charge deconvolution feature within Advion Data Express software automatically calculated the uncharged protein mass from the characteristic multiplycharged spectrum envelope obtained from ESI of biomolecules. The full mass spectra and the deconvolved, uncharged mass for myoglobin is shown in Figure 1.

A total of 14 multiply charged ions of myoglobin were detected with charge state ranging from +9 to +22 (Figure 1A). The uncharged mass of myoglobin is calculated to be 16,951.2 (Figure 1B), within 1 Da of the theoretical mass, 16,950.5.

Learn more about Touch Express at www.advion.com

Figure 1.

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