Effortless DNA library normalization using the ASSIST PLUS pipetting robot equipped with the D-ONE single channel pipetting module

Introduction

Next generation sequencing (NGS) is used for DNA and RNA sequencing in basic, applied and clinical research, as well as diagnostics. NGS was originally called ‘massively parallel sequencing’, referring to its capacity to analyze millions of sequencing reactions at the same time. At the start of the NGS workflow, libraries of similarly sized DNA fragments are created. Normalization of these DNA libraries is then performed, as differences in the concentrations of fragments affect the sequencing results, leading to under- or over-representation of individual fragments.