Subscribe to Newsletter

Evaluation of mAb ADCC Activity through Fc Receptor Affinity Chromatography

Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism of action of anti-tumor antibodies in which the Fcy receptor (FcR) plays a key role. Hence, affinity chromatography using recombinant FcR can deliver valuable information about ADCC activity. Data on the analysis of therapeutic mAbs with the new TSKgel FcR-IIIA-NPR HPLC column are provided.

The N-glycans of antibody Fc domains are known to play an important role in Fc-mediated effector functions. Accordingly, separation patterns of therapeutic antibodies on TSKgel FcR-IIIA-NPR can be correlated to mAb glycoforms. Early eluting peaks (low affinity to Fc receptor) represent mAb glycoforms with low ADCC activity while late eluting peaks represent glycoforms with high ADCC activity.

Read the full article now

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE!

Receive content, products, events as well as relevant industry updates from The Analytical Scientist and its sponsors.
Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:
  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine