Introduction
Dilute and shoot (D/S) is the most common form of sample preparation for the analysis of drugs of abuse in urine. High analyte cutoffs combined with sensitive mass spectrometers allow substantial sample dilution while still reaching desired limits of quantitation. However, this technique presents various issues resulting in increased MS downtime. This poster evaluates the extraction of a range of drugs of abuse from hydrolyzed and nonhydrolyzed urine using a novel flow-through matrix scavenging plate. Specific investigation of matrix component removal in terms of creatinine and urea, salt residue, pigmentation associated with urobillin content and protein removal will be demonstrated.
Experimental
Reagents Standards, ammonium acetate, ammonium formate and formic acid were purchased from Sigma-Aldrich Company ltd. (Gillingham, UK). LC/MS grade solvents were from Honeywell Research Chemicals (Bucharest, Romania). Water (18.2 MΩ.cm) was drawn fresh daily from a Direct-Q 5 water purifier (Merck Millipore, Watford, UK). Urine was kindly donated by healthy human volunteers. Sample Preparation Extractions were performed using a novel flow-through matrix scavenging plate in 96-well format. Non-hydrolyzed or hydrolyzed urine (100 μL) was pipetted into the wells and allowed to incubate if necessary. Acetonitrile (600 μL) was added and mixed using 5x aspirate and dispense steps. Elution was performed using 5 psi positive pressure for 2 minutes.
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