Extraction of 1, α25-di-OH Vitamin D2, 1, α25-di-OH Vitamin D3, 25-OH Vitamin D2 and 25-OH Vitamin D3 from Serum Using ISOLUTE® SLE+ Prior to LC-MS/MS Analysis

contributed by Biotage | 03/28/2014

This application note describes a Supported Liquid Extraction (SLE) protocol for the simultaneous extraction of 25-OH and 1, α25 di-OH-vitamin D2 and D3 metabolites from serum prior to LC-MS/MS analysis.

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE!

Email*

Choose a password*

I have read and understand the Privacy Notice *

Stay up to date with our other newsletters and sponsors information, tailored specifically to the fields you are interested in

Receive communication from The Cannabis Scientist I want to stay up to date with the Mass Spectrometry field I want to stay up to date with the Spectroscopy field

When you click “Subscribe” we will email you a link, which you must click to verify the email address above and activate your subscription. If you do not receive this email, please contact us at [email protected].
If you wish to unsubscribe, you can update your preferences at any point.

Automated cryogen-free monitoring of ‘air toxics’ in ambient air using TD–GC–MS in accordance with US EPA Method TO‑17

11/11/2024 | Contributed by Markes International Ltd

The impact of COVID-19 lockdowns on New York City’s air quality: continuous, cryogen-free on-line monitoring of PAMS in ambient air using hydrogen carrier gas

11/11/2024 | Contributed by Markes International Ltd

Going beyond the requirements of US EPA Method TO-15: Innovative cryogen-free ambient air monitoring of trace-level air toxics at high humidity

11/11/2024 | Contributed by Markes International Ltd

Nitrosamine Analysis in the Pharma Industry: Trends, Challenges, and Future Perspectives

11/14/2024 | Sponsored by LGC

A scalable approach to absolute quantitation in metabolomics

10/31/2024 | Sponsored by Matterworks

Introduction to KnowItAll for Mass Spectrometry

10/29/2024 | Sponsored by Wiley

1.

Fields & Applications Environmental

New Frontiers in Environmental Analysis: Atmospheric Alarms

11/15/2024 | 7 min read

2.

Techniques & Tools Mass Spectrometry

FLiP-MS: A Turn for the Better

11/20/2024 | Henry Thomas | 6 min read

3.

Techniques & Tools Technology

The Analytical Scientist Innovation Awards 2024: 15–11

11/26/2024 | 4 min read

4.

Fields & Applications Clinical

24-Hour One-Shot Pathogen Detector

11/22/2024 | James Strachan | 4 min read

5.

Fields & Applications Environmental

PFAS Remediation: Do We Have the Solutions to the Forever Problem?

10/30/2024 | Diana Aga | 11 min read

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

Unlimited access to ALL articles
News, interviews & opinions from leading industry experts
Receive print (and PDF) copies of The Analytical Scientist magazine