Extraction of 25-hydroxy Vitamin D from Serum Using ISOLUTE® PLD+ prior to LC-MS/MS Analysis

contributed by Biotage | 05/13/2015

This application note describes the extraction of 25-hydroxy vitamin D from serum, prior to LC-MS/MS analysis.

ISOLUTE^® PLD+ Protein and Phospholipid Removal plates offer a substantial improvement in extract cleanliness compared to traditional protein precipitation techniques for bioanalytical sample preparation.

This application note describes a simple, effective ISOLUTE^® PLD+ protocol for the extraction of 25-hydroxy vitamin D from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts.

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