Extraction of a Comprehensive Steroid Panel from Human Serum Using ISOLUTE® SLE+ Prior to LC/MS-MS Analysis

Introduction

This application note describes the extraction of a panel of steroid hormones from human serum using ISOLUTE^® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis. The simple sample preparation procedure delivers clean extracts and high, reproducible recoveries (>75%, RSD <10% ) for all analytes in human serum, with linearity >0.99 in the range 5–5000 pg/mL.

ISOLUTE SLE+ Supported Liquid Extraction plates offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.

Figure 1. Structures of DHEAS, Estradiol and Testosterone.

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