Extraction of Acrylamide from Coffee Using ISOLUTE® SLE+ Prior to LC-MS/MS Analysis

contributed by Biotage | 09/25/2013

This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of acrylamide from coffee using ISOLUTE® SLE+ columns with LC-MS/MS detection.

The method described in this application note achieves high recoveries of acrylamide in coffee. The method is sensitive enough to measure levels as low as 1 ng/mL in coffee (solution), 25 ppm in ground coffee (solid) or 125 ppm in instant coffee (solid, traditional or decaffeinated) and gives good selectivity from what is a challenging matrix.

ISOLUTE SLE+ products provide clean, rapid, robust and efficient extraction solutions for a wide range of analytes.

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