Extraction of Aflatoxins and Ochratoxin from Dried Chili Using ISOLUTE® Myco Prior to LC-MS/MS Analysis

Introduction

This application note describes a solid phase extraction (SPE) protocol for the extraction of a range of mycotoxins from dried chili (pimiento) using ISOLUTE^® Myco with LC-MS/ MS analysis.

Mycotoxins are toxic metabolites produced by fungal molds on food crops. Regulation and legislation for testing of mycotoxin contamination has established which mycotoxins are prevalent on a wide variety of food crops. This application note describes an SPE protocol appropriate for LC-MS/MS analysis of a range of mycotoxins found on chili. The method described in this application note achieves high recoveries of relevant mycotoxins from dried chili (pimiento) with %RSDs and LOQs that meet the requirements set in European Union regulations for measurement of these analytes. ISOLUTE^® Myco solid phase extraction columns provide robust, reliable sample preparation for multiple mycotoxin classes from a wide range of foodstuffs. Using a single, easy to use sample preparation product, along with optimized matrix specific application notes, scientists can prepare diverse food/crop samples for analysis by LC-MS/MS.

Figure 1. Structures of Aflatoxin B1 and Ochratoxin A

Analytes

Aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, ochratoxin A

Sample Preparation Procedure

Format: ISOLUTE^® Myco 60 mg/3 mL Columns (Tabless), part number 150-0006-BG

Sample Pre-treatment

Sample processing: Grind the sample (50 g) with a burr-grinder or equivalent device. Store ground sample in a sealed container at room temperature until required. Extraction: Mix the ground sample (5g) with 80% acetonitrile (aq) (20 mL). Place the sample pre-treatment tube on a shaking table for 30 mins. Transfer the extract to a 50 mL centrifuge tube and centrifuge at 4000 g for 10 minutes. Dilution: Take the supernatant (2 mL), transfer to a new 50 mL centrifuge tube and dilute with water (32 mL). Centrifuge diluted extract at 4000 g for a further 10 minutes.

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