Extraction of Amphetamines and Analogues from Hair Using ISOLUTE® SLE+ Prior to GC/MS Analysis

Introduction

This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization.

This application note describes an effective and efficient ISOLUTE^® SLE+ protocol optimized for 1 mL capacity formats. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 72% with RSDs lower than 10% for all analytes.

In addition, this note describes how to achieve quantitation of all analytes down to 0.2 ng/mg of hair. This complies with the LOQ values for laboratories involved in amphetamine testing, as suggested by the Society of Hair Testing (SoHT). ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquidliquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.

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