Extraction of Thyroid Hormones, T3, rT3 and T4 from Serum

Using EVOLUTE^® EXPRESS AX Plates Prior to LC-MS/MS Analysis

Introduction

This application note describes a polymer based-based strong anion exchange mixed-mode SPE protocol for the extraction of T3, rT3 and T4 from serum prior to LC-MS/MS analysis.

The method described in this application note demonstrates selective extraction of the thyroid hormones T3, rT3 and T4 from serum. 500 μL of serum was extracted using the EVOLUTE EXPRESS AX 30mg fixed well plate format. High reproducible recoveries and extremely low phospholipid content were observed, demonstrating limits of quantitation of 50 pg/mL.