FAMEs analysis method revalidation from helium to hydrogen for GC

contributed by Peak Scientific | 11/12/2014

Changing carrier gas from helium to hydrogen does not always present an opportunity for faster sample analysis. Method revalidation can be simplified by keeping the new method as close to the old method as possible, which will limit changes to sample selectivity and resolution whilst maintaining the retention times of analytes.

Fatty acid methyl esters (FAMEs) analysis is an important method used across a range of product analyses to characterise fat content. Analysis of FAMEs is conducted in a wide range of products of biological origin including foodstuffs, biodiesels and microbes. The quantification of FAMEs is usually conducted using gas chromatography with flame ionisation detection (GC-FID) using a highly polar column.

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