Introduction
The normalized metabolite concentrations of epinephrine and norepinephrine precursors have common utility in clinical diagnostics related to neuroendocrine tumors. Of particular interest are the selected metabolites metanephrine and normetanephrine. The challenges in population screening for these metabolites are time, cost and efficacy of sample preparation strategies required for trace level determinations (pg/mL-ng/mL levels) by liquid chromatography-tandem mass spectrometry.
Figure 1. Structure of metanephrine and normetanephrineA new solid phase extraction technology has been developed to reduce the time associated with sample preparation methods by eliminating steps in the extraction workflow. Leveraging this technology, a method protocol was developed using a load, wash and elute approach. The method was evaluated for relative recovery, repeatability and linearity of analyte response with concentration. Recoveries exceeding 8o% were achieved for both metanephrine and normetanephrine. EVOLUTE EXPRESS CX ion exchange SPE materials offer an efficient alternative to traditional ion exchange SPE plates for bioanalytical sample preparation, providing high analyte recoveries and significantly reduced sample preparation time.
Analytes
Metanephrine, normetanephrine.
Sample Preparation Procedure
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