Cookies

Like most websites The Analytical Scientist uses cookies. In order to deliver a personalized, responsive service and to improve the site, we remember and store information about how you use it. Learn more.

Fluorodeoxyglucose Radiotracer Analysis

Fluorodeoxyglucose, [<sup>18</sup>F]FDG, Radiotracer Analysis

Introduction

In PET imaging, the radio-labelled tracer 2-deoxy-2-(18F)fluoro-D-glucose, also called [18F]-Fluorodeoxyglucose, [18F]FDG or FDG is often used for the assessment of glucose metabolism in the heart, lungs, and the brain as well as for imaging tumors in oncology. The 109.8 minute half-life of [18F]FDG makes rapid and automated chemistry a requirement; therefore [18F]FDG is produced in a cyclotron in close vicinity of the PET facility. Prior to injection of [18F]FDG into a patient, it is necessary to perform a purity check and determine the actual concentration of the unwanted by-products: 2-fluoro-2-deoxy-D-mannose (FDM) and 2-chloro-2-deoxy-D-glucose (CDG). HPLC-ECD is the industry standard for this important test due to its ability to selectively detect [18F]FDG, FDM and CDG at very low concentration levels. Compendial methods based on ECD are described in both the US Pharmacopeia (USP) and European Pharmacopoeia (EP). These EP and USP methods are similar and based on High Performance Anion-Exchange Chromatography (HPAEC) in combination with Pulsed Amperometric Detection (PAD). In this application note Antec’s ALEXYS HPAEC-PAD analyzer is used following the conditions described in the official USP and EP monographs. The ALEXYS analyzer for FDG is based on the DECADE Elite electrochemical detector in combination with a CarboPac PA20 analytical column, which allows for fast analysis of FDG and its by-products in less than 8 minutes.

Read the full article now

Log in or register to read this article in full and gain access to The Analytical Scientist’s entire content archive. It’s FREE and always will be!

Login if you already created an account

Or register now - it’s free and always will be!

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine
Register

Or Login as a Guest or via Social Media

Related Application Notes

Techniques & Tools Gas Chromatography

What is thermal desorption?

| Contributed by Markes

Generic Analyses of Different Monoclonal Antibodies with RP-(U)HPLC

| Contributed by YMC

Dubai Police uses TEA by Ellutia for explosives identification

| Contributed by Ellutia

Newsletter

Send me the latest from The Analytical Scientist.

Sign up now

Register to The Analytical Scientist

Register to access our FREE online portfolio, request the magazine in print and manage your preferences.

You will benefit from:

  • Unlimited access to ALL articles
  • News, interviews & opinions from leading industry experts
  • Receive print (and PDF) copies of The Analytical Scientist magazine

Register