GC-TOFMS Analysis of Urine Extract Samples

Introduction

The interest in comprehensive analysis of the metabolome has increased dramatically in the past decade. No single analytical technique can be used to accomplish this comprehensive study, but using various techniques in a complimentary way can make the task easier and enhance the chances of success. Gas chromatography coupled with mass spectrometry (GC-MS) is one of the techniques that are attractive to scientists involved in studies of the metabolome, due to the very good selectivity and sensitivity that it can provide. The only drawback was the lack of analysis speed. The addition of a Time-of-Flight Mass Spectrometer (TOFMS) with its high-speed capabilities overcame this difficulty. With acquisition speeds of up to 500 spectra/second, lack of spectral skewing, and unique Peak Find and Deconvolution algorithms, the LECO Pegasus GC-TOFMS facilitates the analysis of thousands of samples every year.