μPAC™ column robustness in bottom-up proteomics
contributed by Pharma Fluidics |
μPAC™ column robustness in bottom-up proteomics
Introduction
Being one of the main workhorses in today’s analytical research, the output of many laboratories is dominated by the amount of time that LC-MS systems are running smoothly. With a lot of determining factors, it is often quite a challenge to keep these systems up and running. Next to technical issues that can occur with the LC-MS system’s hard- and software, LC column failure is one of the most frequent causes of LC-MS system down time. Typical observations of LC column failure are an increase in column backpressure, deviating peak shapes or gradual shifts in retention time.
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