Navigating the Evolution of Light-Sheet Microscopy Through Time and Technology

Journey through the history of scientific exploration, where light-sheet microscopy meets the complexities of life sciences. Over the past three decades, microscopic techniques have advanced remarkably, fueled by the innovation of scientific CMOS cameras.

contributed by Hamamatsu | 03/06/2025

Light microscopy is a core technology in life science research. Since its early days, it has evolved with a wide range of microscopic techniques, driven by the rapid development of optoelectronic detectors and the widespread availability of laser-based light sources.

This development is clear when observing the distinctive design of light-sheet microscopes, which varies greatly from the standard microscope design that has remained for centuries.

The fundamental concept underlying all light-sheet microscopes is that the sample is illuminated with a thin sheet of light (hence the name), typically perpendicular to the observation axis. This decoupling of the illumination and the detection offers greater freedom in design compared to typical epi- or transillumination designs.

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