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Non-targeted profiling of polar metabolites in human plasma

The fascinating aspect about non-targeted LC analysis is the presence of unknown compounds, for example in biomarker research. A particular challenge for chromatography is then to find the balance between sensitivity and covering a broad spectrum of substances. Hydrophilic liquid chromatography (HILIC) is therefore especially suitable for such analyses containing polar compounds. It is a perfect match for mass spectrometry (MS) and has very good chromatographic performance. With this combination, non-targeted analysis is well suited to the screening of polar metabolites in human plasma.

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