The fascinating aspect about non-targeted LC analysis is the presence of unknown compounds, for example in biomarker research. A particular challenge for chromatography is then to find the balance between sensitivity and covering a broad spectrum of substances. Hydrophilic liquid chromatography (HILIC) is therefore especially suitable for such analyses containing polar compounds. It is a perfect match for mass spectrometry (MS) and has very good chromatographic performance. With this combination, non-targeted analysis is well suited to the screening of polar metabolites in human plasma.
This application note shows the non-targeted screening of polar metabolites in human plasma using a bioinert YMC-Accura Triart Diol-HILIC column. The bioinert coated stainless-steel hardware is essential to achieve the highest sensitivity and chromatographic resolution of endogenous isomers. For this analysis proteins contained in the plasma were precipitated with acetonitrile (3:1 v/v). After decanting and drying the sample, it was re-dissolved with acetonitrile/water (70/30) to the original concentration.
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