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Oh so sweet - Quantification of steviol glycosides in Stevia samples with RP-HPLC

Summary

Steviol glycosides are the main sweetening compounds in Stevia rebaudiana and can be used as natural sugar substitutes. This method provides a fast determination of six steviol glycosides using the AZURA® HPLC Plus System. With a fast separation under 10 min and an optimized gradient, the developed method is suitable for a fast quality control of stevia products. Furthermore, the robustness for this method was validated using DryLab simulation software.

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