Profiling of Citrus Oils and Determination of Furocoumarins in Citrus Oils Using the Agilent 1290 Infinity 2D-LC Solution

contributed by Agilent Technologies | 02/18/2014

Comprehensive two-dimensional liquid chromatography (LC×LC) using the Agilent 1290 Infinity 2D-LC Solution with DAD and MS detection is applied for the analysis of citrus oils. The developed method uses normal phase liquid chromatography in the first dimension and reversed-phase liquid chromatography in the second dimension. This combination provides good orthogonality and is valuable for profiling various citrus oils. The method also enables the detection of (potentially) carcinogenic furocoumarins in citrus oil samples.

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