Redox titration of vitamin C in orange juice using OMNIS system

Vitamin C is an important antioxidant that is ubiquitous in orange juice. The determination of the vitamin C content in orange juice is therefore of importance.

With the OMNIS system a fast and accurate determination of vitamin C in orange juice by a potentiometric titration with the double Pt-sheet electrode is realized. The vitamin C content is determined using iodine as titrant.

Sample

Orange juice

Sample preparation

No sample preparation is required.

Analysis

Sample 50 mL orange juice is pipetted into the titration vessel and placed on the rack. Just before the titration 2 mL glyoxal solution is automatically added to the sample. After a reaction time of 5 minutes, 5 mL sulfuric acid is automatically added and the solution is titrated with c(I₂) = 0.01 mol/L until after the equivalence point using the double Pt-sheet electrode.

>> Download the full Application Note as PDF

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